PepShop

PepShop
University of Illinois at Urbana-Champaign

HELP

Input
Output

PepShop is an unified web-portal of neuropeptide and prohormone (or proprotein) information. Sequence and mass spectra data is integrated from cleavage prediction and other bionformatics tools mainly from NeuroPred and SwePep with information and tools from UniProt, NCBI.

Query Prohormone Database

Accession Number

Prohormone Symbol

Species

Monoisotopic mass (in Daltons)

Mass tolerance (±)

Prohormone sequence

Proteomics MS/MS Tools

MS/MS spectrum search

X!Tandem Crux OMSSA

Predict Fragment Ions from Sequence

Please paste sequence in submission box

Parameters

Precursor charge state
Mass type	monoisotopic average
Ion types	a ions b ions c ions x ions y ions z ions
Neutral mass losses	Ammonia loss (-17Da) Water loss (-18Da)

Bioinformatics Tools: NeuroPred, BLAST and Muscle

Please submit sequence in fasta format

or upload file

NeuroPred Parameters

Model Selection

Post-Translational Modifications (PTMs)
Trim C-terminal K and R
Most Common PTMs	Less Common PTMs
Amidation	O-linked Glycosylation of S	N-linked Glycosylation of S	Bromination of W
Pyroglutamination	O-linked Glycosylation of T	N-linked Glycosylation of T	Methylation of E
Acetylation	Dipeptidase	Hydroxylation of P	Methylation of H
Sulfation of Y	Carboxylation of E	Phosphorylation of S	Methylation of K
	DiAcetylation	Phosphorylation of T	Methylation of R

BLAST Parameters

Database Selection

Questions or comments: Sandra Rodriguez Zas (rodrgzzs@illinois.edu)
	This Database is supported by NIH/NIDA Grants: R21 DA027548 and P30 DA 018310.